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KMID : 0881720170320060470
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Journal of Food Hygiene and Safety
2017 Volume.32 No. 6 p.470 ~ p.476
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A Study on the Safety of Mycotoxins in Grains and Commonly Consumed Foods
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Kim Jae-Kwan
Kim Young-Sug
Lee Chang-Hee
Seo My-Young
Jang Mi-Kyung
Ku Eun-Jung
Park Kwang-Hee
Yoon Mi-Hye
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Abstract
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The purpose of this study was to investigate and evaluate the safety of the grains, nut products, beans and oilseeds being sold in Gyeonggi province by analyzing mycotoxins. A multi-mycotoxins analysis method based on LC-MS/MS was validated and applied for the determination of eight mycotoxins, including aflatoxins (B1, B2, G1 and G2), fumonisins (B1, B2), zearalenone and ochratoxcin A in 134 samples. The limit of detection (LOD) and limit of quantitation (LOQ) for the eight mycotoxins ranged from 0.14 to 8.25 μg/kg and from 1.08 to 7.21 μg/kg, respectively. Recovery rates of mycotoxins were determined in the range of 61.1 to 97.5% with RSD of 1.0~14.5% (n=3). Fumonisin B1, B2, zearalenone, and ochratoxin A were detected in 22 samples, indicating that 27% of grains, 12.5% of beans and 11.8% of oilseeds were contaminated. Fumonisin and zearalenone were detected simultaneously in 2 adlays and 3 sorghums. Fumonisin B1 and B2 were detected simultaneously in most samples whereas fumonisin B1 was detected in 1 adlay, 1 millet and 1 sesame sample. The average detected amount of fumonisin was 49.3 μg/kg and 10.1 μg/kg for grains and oilseeds, respectively. The average detected amount of zearalenone was 1.9 μg/kg and 1.5 μg/kg for grains and beans, respectively. In addition, the average amount of ochratoxin A was 0.08 μg/kg for grains. The calculated exposure amounts of fumonisin, zeralenone and ochratoxin A for grains, beans and oilseeds were below the PMTDI/PTWI.
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KEYWORD
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Mycotoxin , Aflatoxin , Fumonisin , Zearalenone , Ochratoxin A
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